Contactin 2 homophilic adhesion structure and conformational plasticity

Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
Structure (2023 Nov)

doi: 10.1016/j.str.2023.10.012

Submitted to SASBDB: 2022 Apr 26 Published in SASBDB: 2023 Nov 22

SASDNP8 – High mannose contactin-2 immunoglobulin domains1-6, 1μM Sample: Contactin-2 dimer, 150 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 5.1 nm Dmax 20.0 nm VolumePorod 140 nm3

SASDNQ8 – High mannose contactin 2 immunoglobulin domains1-6, 3μM Sample: Contactin-2 dimer, 150 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 5.3 nm Dmax 20.0 nm VolumePorod 300 nm3

SASDNR8 – High mannose contactin-2 immunoglobulin domains1-6, 6μM Sample: Contactin-2 dimer, 150 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 5.4 nm Dmax 21.0 nm VolumePorod 325 nm3

SASDNS8 – High mannose contactin-2 immunoglobulin domains1-6, 13μM Sample: Contactin-2 dimer, 150 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 5.5 nm Dmax 21.4 nm VolumePorod 350 nm3

SASDNT8 – High mannose contactin-2 immunoglobulin domains1-6, 26μM Sample: Contactin-2 dimer, 150 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 6.0 nm Dmax 30.0 nm VolumePorod 380 nm3

SASDNU8 – High mannose contactin-2 immunoglobulin domains1-6, 52μM Sample: Contactin-2 dimer, 150 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 6.1 nm Dmax 32.0 nm VolumePorod 408 nm3

SASDNV8 – Complex glycan contactin-2 immunoglobulin domains1-6, 1μM Sample: Contactin-2 dimer, 157 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 5.6 nm Dmax 25.0 nm VolumePorod 230 nm3

SASDNW8 – Complex glycan contactin-2 immunoglobulin domains1-6, 3μM Sample: Contactin-2 dimer, 157 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 5.4 nm Dmax 25.0 nm VolumePorod 185 nm3

SASDNX8 – Complex glycan contactin-2 immunoglobulin domains1-6, 5μM Sample: Contactin-2 dimer, 157 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 5.5 nm Dmax 22.0 nm VolumePorod 353 nm3

SASDNY8 – Complex glycan contactin-2 immunoglobulin domains1-6, 11μM Sample: Contactin-2 dimer, 157 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 5.5 nm Dmax 22.0 nm VolumePorod 365 nm3

SASDNZ8 – Complex glycan contactin-2 immunoglobulin domains1-6, 24μM Sample: Contactin-2 dimer, 157 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 5.6 nm Dmax 22.0 nm VolumePorod 390 nm3

SASDN29 – Complex glycan contactin-2 immunoglobulin domains1-6, 51μM Sample: Contactin-2 dimer, 157 kDa Mus musculus protein Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 7 RgGuinier 5.8 nm Dmax 32.0 nm VolumePorod 412 nm3

SASDN39 – Complex glycan contactin-2 ectodomain, 1 μM Sample: Contactin-2 trimer, 389 kDa Mus musculus protein Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 9.3 nm Dmax 45.0 nm VolumePorod 780 nm3

SASDN49 – Complex glycan contactin-2 ectodomain, 2 μM Sample: Contactin-2 trimer, 389 kDa Mus musculus protein Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 10.3 nm Dmax 60.0 nm VolumePorod 1290 nm3

SASDN59 – Complex glycan contactin-2 ectodomain, 3 μM Sample: Contactin-2 trimer, 389 kDa Mus musculus protein Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 11.2 nm Dmax 60.0 nm VolumePorod 1540 nm3

SASDN69 – Complex glycan contactin-2 ectodomain, 6 μM Sample: Contactin-2 trimer, 389 kDa Mus musculus protein Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 11.0 nm Dmax 70.0 nm VolumePorod 1900 nm3

SASDN79 – Complex glycan contactin-2 ectodomain, 12 μM Sample: Contactin-2 trimer, 389 kDa Mus musculus protein Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 11.3 nm Dmax 66.0 nm VolumePorod 1820 nm3

SASDN89 – Complex glycan contactin-2 ectodomain, 23 μM Sample: Contactin-2 trimer, 389 kDa Mus musculus protein Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 12.5 nm Dmax 65.0 nm VolumePorod 2044 nm3

SASDN99 – Complex glycan contactin-2 ectodomain, 50 μM Sample: Contactin-2 trimer, 389 kDa Mus musculus protein Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17 RgGuinier 14.2 nm Dmax 65.0 nm VolumePorod 2190 nm3