LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers

Joseph S, Schnicker N, Xu Z, Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
SSRN Electronic Journal ()

doi: 10.2139/ssrn.4151031

Submitted to SASBDB: 2022 Apr 5 Published in SASBDB: 2024 Apr 5

SASDNF8 – LARGE xylosyl- and glucuronyltransferase 1 (LARGE1dTM) dimer Sample: Xylosyl- and glucuronyltransferase LARGE1 dimer, 179 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18 RgGuinier 4.4 nm Dmax 18.4 nm VolumePorod 264 nm3

SASDNG8 – LARGE xylosyl- and glucuronyltransferase 1 (LARGE1dTM) dimer treated with PNGase F enzyme Sample: Xylosyl- and glucuronyltransferase LARGE1 dimer, 179 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18 RgGuinier 4.3 nm Dmax 18.0 nm

SASDNH8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer Sample: Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18 RgGuinier 4.2 nm Dmax 16.5 nm

SASDNJ8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer treated with PNGase F enzyme Sample: Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18 RgGuinier 4.1 nm Dmax 15.9 nm VolumePorod 231 nm3