Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii.

Lyons NS, Bogner AN, Tanner JJ, Sobrado P
Biochemistry (2022 Oct 31)

PMID: 36314559 doi: 10.1021/acs.biochem.2c00493

Submitted to SASBDB: 2022 Apr 26 Published in SASBDB: 2022 Nov 7

SASDNA9 – Acinetobacter baumannii putrescine N-hydroxylase, 1 mg/mL Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14 RgGuinier 4.1 nm Dmax 15.0 nm VolumePorod 394 nm3

SASDNB9 – Acinetobacter baumannii putrescine N-hydroxylase, 3 mg/mL Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14 RgGuinier 4.1 nm Dmax 15.0 nm VolumePorod 390 nm3

SASDNC9 – Acinetobacter baumannii putrescine N-hydroxylase, 5 mg/mL Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14 RgGuinier 4.2 nm Dmax 15.0 nm VolumePorod 390 nm3

SASDND9 – Acinetobacter baumannii putrescine N-hydroxylase, 6 mg/mL Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14 RgGuinier 4.2 nm Dmax 15.4 nm VolumePorod 390 nm3

SASDNE9 – Acinetobacter baumannii putrescine N-hydroxylase, 8 mg/mL Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14 RgGuinier 4.2 nm Dmax 15.5 nm VolumePorod 390 nm3

SASDNF9 – Acinetobacter baumannii putrescine N-hydroxylase, 9 mg/mL Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14 RgGuinier 4.2 nm Dmax 15.0 nm VolumePorod 396 nm3