Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions.

Ohara N, Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K
J Am Chem Soc (2022 Dec 21)

PMID: 36541447 doi: 10.1021/jacs.2c09537

Submitted to SASBDB: 2022 Apr 29 Published in SASBDB: 2022 Dec 22

SASDPV2 – original TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins) Sample: Fusion protein of LSm and MyoX-coil, 1066 kDa Artificial protein protein Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23 RgGuinier 9.1 nm Dmax 22.7 nm

SASDPW2 – mTIP60-Ba (metal-ion induced 60-mer complex of TIP60 (K67E) mutant with barium ions) Sample: TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions), 1066 kDa Artificial protein protein Barium ion 0, 8 kDa Buffer: 25 mM HEPES, 100 mM NaCl, 5% glycerol, 5 mM BaCl2, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23 RgGuinier 9.6 nm Dmax 21.8 nm

SASDPX2 – TIP60 (K67E) mutant (dimer) with EDTA Sample: TIP60 (K67E) mutant with EDTA dimer, 36 kDa Artificial protein protein Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23 RgGuinier 3.7 nm Dmax 12.0 nm