Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein.

Andreeva EA, Niziński S, Wilson A, Levantino M, De Zitter E, Munro R, Muzzopappa F, Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
Biophys J 121(15):2849-2872 (2022 Aug 2)

PMID: 35794830 doi: 10.1016/j.bpj.2022.07.004

Submitted to SASBDB: 2022 May 6 Published in SASBDB: 2022 Sep 5

SASDNY9 – dark-adapted Orange Carotenoid Protein-wtCtag at 0.7 mg/mL Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 2.4 nm Dmax 6.7 nm VolumePorod 55 nm3

SASDNZ9 – dark-adapted Orange Carotenoid Protein-wtCtag at 3.5 mg/mL Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 2.8 nm Dmax 9.1 nm VolumePorod 77 nm3

SASDP22 – dark-adapted Orange Carotenoid Protein-wtCtag at 10.5 mg/mL Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 2.8 nm Dmax 12.7 nm VolumePorod 85 nm3

SASDP32 – dark-adapted Orange Carotenoid Protien-wtCtag at 23 mg/mL Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 2.8 nm Dmax 13.0 nm VolumePorod 89 nm3

SASDP42 – dark-adapted Orange Carotenoid Protein-R27LNtag at 0.7 mg/mL Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 2.2 nm Dmax 8.0 nm VolumePorod 44 nm3

SASDP52 – dark-adapted Orange Carotenoid Protein-R27LNtag at 3.5 mg/mL Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 2.4 nm Dmax 8.0 nm VolumePorod 61 nm3

SASDP62 – dark-adapted Orange Carotenoid Protein-R27LNtag at 10.5 mg/mL Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 2.3 nm Dmax 8.0 nm VolumePorod 58 nm3

SASDP72 – dark-adapted Orange Carotenoid Protein-R27LNtag at 14 mg/mL Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 2.3 nm Dmax 8.0 nm VolumePorod 58 nm3

SASDP82 – light-adapted Orange Carotenoid Protein-wtCtag at 0.7 mg/mL Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 2.9 nm Dmax 14.0 nm VolumePorod 63 nm3

SASDP92 – light-adapted Orange Carotenoid Protein-wtCtag at 3.5 mg/mL Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 3.4 nm Dmax 17.0 nm VolumePorod 78 nm3

SASDPA2 – light-adapted Orange Carotenoid Protein-wtCtag at 10.5 mg/mL Sample: Orange carotenoid-binding protein, 105 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 4.4 nm Dmax 31.0 nm VolumePorod 140 nm3

SASDPB2 – light-adapted Orange Carotenoid Protein-R27LNtag at 0.7 mg/mL Sample: Orange carotenoid-binding protein, 105 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 3.3 nm Dmax 15.0 nm VolumePorod 70 nm3

SASDPC2 – light-adapted Orange Carotenoid Protein-R27LNtag at 3.5 mg/mL Sample: Orange carotenoid-binding protein, 105 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 3.4 nm Dmax 19.0 nm VolumePorod 70 nm3

SASDPD2 – light-adapted Orange Carotenoid Protein-R27LNtag at 10.5 mg/mL Sample: Orange carotenoid-binding protein, 105 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23 RgGuinier 4.8 nm Dmax 26.0 nm VolumePorod 120 nm3