Asymmetric horseshoe-like assembly of peroxisomal yeast oxalyl-CoA synthetase.

Bürgi J, Lill P, Giannopoulou EA, Jeffries CM, Chojnowski G, Raunser S, Gatsogiannis C, Wilmanns M
Biol Chem (2023 Jan 26)

PMID: 36694962 doi: 10.1515/hsz-2022-0273

Submitted to SASBDB: 2022 Jun 1 Published in SASBDB: 2023 Jan 27

SASDPV3 – Wild type oxalyl-CoA synthetase Pcs60p (2.5 mg/ml) - hexamer-tetramer equilibrium Sample: Oxalate--CoA ligase, 363 kDa Saccharomyces cerevisiae (strain … protein Buffer: 50 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 18 RgGuinier 4.7 nm Dmax 13.2 nm VolumePorod 565 nm3

SASDPW3 – Wild type oxalyl-CoA synthetase Pcs60p (0.5 mg/ml) - tetramer-hexamer equilibrium Sample: Oxalate--CoA ligase, 363 kDa Saccharomyces cerevisiae (strain … protein Buffer: 50 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 18 RgGuinier 4.5 nm Dmax 13.7 nm VolumePorod 432 nm3

SASDPX3 – Point mutant K352D oxalyl-CoA synthetase Pcs60p (2.5 mg/ml) - dimer Sample: Oxalate--CoA ligase (K352D) dimer, 121 kDa Saccharomyces cerevisiae (strain … protein Buffer: 50 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 18 RgGuinier 3.3 nm Dmax 11.1 nm VolumePorod 163 nm3

SASDPY3 – Point mutant K352D oxalyl-CoA synthetase Pcs60p (0.5 mg/ml) - dimer Sample: Oxalate--CoA ligase (K352D) dimer, 121 kDa Saccharomyces cerevisiae (strain … protein Buffer: 50 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 18 RgGuinier 3.3 nm Dmax 10.2 nm VolumePorod 194 nm3