Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro.

Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
Sci Adv 8(49):eadd2191 (2022 Dec 9)

PMID: 36490335 doi: 10.1126/sciadv.add2191

Submitted to SASBDB: 2022 May 15 Published in SASBDB: 2022 Dec 12

SASDPY2 – SARS-CoV-2 non-structural protein 7-8 (nsp7-8) polyprotein monomer Sample: Replicase polyprotein 1ab monomer, 31 kDa Severe acute respiratory … protein Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15 RgGuinier 2.5 nm Dmax 8.8 nm VolumePorod 50 nm3

SASDPZ2 – SARS-CoV-2 non-structural protein 7-8 (nsp7-8) polyprotein dimer Sample: Replicase polyprotein 1ab dimer, 63 kDa Severe acute respiratory … protein Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15 RgGuinier 3.3 nm Dmax 11.8 nm VolumePorod 110 nm3

SASDP23 – SARS-CoV-2 non-structural protein 7-11 (nsp7-11) polyprotein monomer Sample: Replicase polyprotein 1a monomer, 60 kDa Severe acute respiratory … protein Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14 RgGuinier 3.5 nm Dmax 15.6 nm VolumePorod 102 nm3

SASDP33 – SARS-CoV-2 non-structural protein 7-11 (nsp7-11) polyprotein dimer Sample: Replicase polyprotein 1a dimer, 119 kDa Severe acute respiratory … protein Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14 RgGuinier 4.6 nm Dmax 19.1 nm VolumePorod 203 nm3