Allosteric autoregulation of DNA binding via a DNA-mimicking protein domain: a biophysical study of ZNF410-DNA interaction using small angle X-ray scattering.

Kaur G, Ren R, Hammel M, Horton JR, Yang J, Cao Y, He C, Lan F, Lan X, Blobel GA, Blumenthal RM, Zhang X, Cheng X
Nucleic Acids Res (2023 Jan 20)

PMID: 36660822 doi: 10.1093/nar/gkac1274

Submitted to SASBDB: 2022 Sep 17 Published in SASBDB: 2022 Dec 22

SASDQF5 – Zinc finger protein 410 (ZNF410 full length) Sample: Zinc finger protein 410 monomer, 52 kDa Homo sapiens protein Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30 RgGuinier 3.6 nm Dmax 12.3 nm VolumePorod 108 nm3

SASDQG5 – DNA (Zinc finger protein 410 recognition sequence) Sample: DNA (Zinc finger protein 410 recognition sequence) monomer, 11 kDa DNA Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30 RgGuinier 1.8 nm Dmax 5.8 nm VolumePorod 16 nm3

SASDQH5 – Zinc finger protein 410 (ZNF410 full length) bound to DNA Sample: Zinc finger protein 410 monomer, 52 kDa Homo sapiens protein DNA (Zinc finger protein 410 recognition sequence) monomer, 11 kDa DNA Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30 RgGuinier 4.4 nm Dmax 14.3 nm VolumePorod 76 nm3

SASDQJ5 – Zinc finger protein 410 (ZNF410): N-terminal region with 1-5 zinc fingers Sample: Zinc finger protein 410 monomer, 40 kDa Homo sapiens protein Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30 RgGuinier 3.2 nm Dmax 10.7 nm VolumePorod 78 nm3

SASDQK5 – Zinc finger protein 410 (ZNF410): N-terminal region with 1-5 zinc fingers bound to DNA Sample: DNA (Zinc finger protein 410 recognition sequence) monomer, 11 kDa DNA Zinc finger protein 410 monomer, 40 kDa Homo sapiens protein Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Apr 25 RgGuinier 3.1 nm Dmax 14.1 nm VolumePorod 63 nm3

SASDQL5 – Zinc finger protein 410 (ZNF410): C-terminal region with 1-5 zinc fingers Sample: Zinc finger protein 410 monomer, 29 kDa Homo sapiens protein Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30 RgGuinier 3.0 nm Dmax 9.6 nm VolumePorod 58 nm3

SASDQM5 – Zinc finger protein 410 (ZNF410): C-terminal region with 1-5 zinc fingers bound to DNA Sample: DNA (Zinc finger protein 410 recognition sequence) monomer, 11 kDa DNA Zinc finger protein 410 monomer, 29 kDa Homo sapiens protein Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30 RgGuinier 3.1 nm Dmax 11.9 nm VolumePorod 52 nm3