Two interaction surfaces between XPA and RPA organize the preincision complex in nucleotide excision repair

Kim M, Kim H, D’Souza A, Gallagher K, Jeong E, Topolska-Wós A, Ogorodnik Le Meur K, Tsai C, Tsai M, Kee M, Tainer J, Yeo J, Chazin W, Schärer O
Proceedings of the National Academy of Sciences 119(34) (2022 Aug 15)

doi: 10.1073/pnas.2207408119

Submitted to SASBDB: 2022 May 27 Published in SASBDB: 2022 Jun 2

SASDPZ3 – Complex of XPA1-239 and RPAΔ32NΔ70N complex engaged on 3’ss-ds DNA junction NER substrate Sample: DNA repair protein complementing XP-A cells monomer, 27 kDa Homo sapiens protein Replication protein A 70 kDa DNA-binding subunit monomer, 49 kDa Homo sapiens protein Replication protein A 32 kDa subunit monomer, 25 kDa Homo sapiens protein Replication protein A 14 kDa subunit monomer, 14 kDa Homo sapiens protein 3-prime ss-ds DNA junction NER model substrate monomer, 17 kDa DNA Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 2% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Mar 4 RgGuinier 4.3 nm Dmax 14.7 nm VolumePorod 189 nm3

SASDP24 – Complex of XPA1-239 and RPAΔ32NΔ70N complex engaged on 5’ss-ds DNA junction NER substrate Sample: Replication protein A 14 kDa subunit monomer, 14 kDa Homo sapiens protein DNA repair protein complementing XP-A cells monomer, 27 kDa Homo sapiens protein Replication protein A 70 kDa DNA-binding subunit monomer, 49 kDa Homo sapiens protein Replication protein A 32 kDa subunit monomer, 25 kDa Homo sapiens protein 5-prime ss-ds DNA junction NER model substrate monomer, 17 kDa DNA Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 2% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Mar 4 RgGuinier 4.6 nm Dmax 16.5 nm VolumePorod 220 nm3