X‐ray structure and function of fibronectin domains two and three of the neural cell adhesion molecule L1

Guédez G, Loers G, Jeffries C, Kozak S, Meijers R, Svergun D, Schachner M, Löw C
The FASEB Journal 37(3) (2023 Feb 21)

doi: 10.1096/fj.202201511R 		Submitted to SASBDB: 2022 Jul 26
Published in SASBDB:

SASDPB6 – Fibronectin 2 and 3 of cell adhesion molecule L1 (L1CAM) expressed in HEK293S GnTI-

Neural cell adhesion molecule L1 experimental SAS data
DAMMIN model
Sample: Neural cell adhesion molecule L1 monomer, 24 kDa Homo sapiens protein
Buffer: 50 mM MES pH 6.5, 150 mM NaCl, 5% glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 May 11
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 68 nm3

SASDPC6 – Fibronectin 2 and 3 of cell adhesion molecule L1 (L1CAM) expressed in HEK293F

Neural cell adhesion molecule L1 experimental SAS data
DAMMIN model
Sample: Neural cell adhesion molecule L1 monomer, 24 kDa Homo sapiens protein
Buffer: 50 mM MES pH 6.5, 150 mM NaCl, 5% glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 May 11
RgGuinier 2.9 nm
Dmax 9.9 nm
VolumePorod 72 nm3

SASDPD6 – Fibronectin 2 and 3 of cell adhesion molecule L1 (L1CAM) expressed in HEK293F cells with kifunensine

Neural cell adhesion molecule L1 experimental SAS data
CORAL model
Sample: Neural cell adhesion molecule L1 monomer, 24 kDa Homo sapiens protein
Buffer: 50 mM MES pH 6.5, 150 mM NaCl, 5% glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 May 11
RgGuinier 2.9 nm
Dmax 9.6 nm
VolumePorod 72 nm3