New insights into P2X7 receptor regulation: Ca²⁺-calmodulin and GDP bind to the soluble P2X7 ballast domain.

Sander S, Müller I, Alai MG, Nicke A, Tidow H
J Biol Chem :102495 (2022 Sep 14)

PMID: 36115462 doi: 10.1016/j.jbc.2022.102495

Submitted to SASBDB: 2022 Jun 14 Published in SASBDB: 2022 Jun 24

SASDPE4 – P2X7 ballast domain Sample: P2X purinoceptor 7 trimer, 74 kDa Homo sapiens protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4 RgGuinier 3.1 nm Dmax 10.5 nm VolumePorod 94 nm3

SASDPD4 – Ca2+-calmodulin Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4 RgGuinier 2.1 nm Dmax 6.9 nm VolumePorod 23 nm3

SASDPF4 – P2X7 ballast domain in complex with Ca2+-calmodulin Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein P2X purinoceptor 7 monomer, 25 kDa Homo sapiens protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4 RgGuinier 3.2 nm Dmax 13.5 nm VolumePorod 73 nm3