Structural basis of myelin-associated glycoprotein adhesion and signalling.

Pronker MF, Lemstra S, Snijder J, Heck AJ, Thies-Weesie DM, Pasterkamp RJ, Janssen BJ
Nat Commun 7:13584 (2016 Dec 6)

PMID: 27922006 doi: 10.1038/ncomms13584

Submitted to SASBDB: 2016 Jul 24 Published in SASBDB: 2016 Dec 13

SASDB55 – Glycosylated myelin-associated glycoprotein full extracellular domain (immunoglobulin domains 1-5) Sample: Myelin-associated glycoprotein Ig domains 1-5 dimer, 108 kDa Mus musculus protein Buffer: 25 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11 RgGuinier 6.8 nm Dmax 23.8 nm VolumePorod 177 nm3

SASDB26 – Glycosylated myelin-associated glycoprotein full extracellular domain (Ig1-5) I473E mutant Sample: Myelin-associated glycoprotein (20-508; I473E mutant) monomer, 54 kDa Mus musculus protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11 RgGuinier 6.0 nm Dmax 20.6 nm VolumePorod 117 nm3

SASDB36 – Glycosylated myelin-associated glycoprotein full extracellular domain (Ig1-5) N406Q mutant Sample: Myelin-associated glycoprotein (20-508; N406Q mutant) monomer, 54 kDa Mus musculus protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 5 RgGuinier 7.3 nm Dmax 25.6 nm VolumePorod 193 nm3

SASDB46 – Glycosylated Myelin-associated glycoprotein immunoglobulin domains 1-3 Sample: Myelin-associated glycoprotein Ig domains 1-3 monomer, 35 kDa Mus musculus protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11 RgGuinier 3.9 nm Dmax 13.0 nm VolumePorod 59 nm3

SASDBF6 – Deglycosylated myelin-associated glycoprotein full extracellular domain (immunoglobulin domains 1-5) Sample: Myelin-associated glycoprotein Ig domains 1-5 dimer, 108 kDa Mus musculus protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11 RgGuinier 7.3 nm Dmax 25.5 nm VolumePorod 166 nm3

SASDB56 – Deglycosylated myelin-associated glycoprotein full extracellular domain (Ig 1-5) I473E mutant Sample: Myelin-associated glycoprotein (20-508; I473E mutant) monomer, 54 kDa Mus musculus protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11 RgGuinier 6.0 nm Dmax 21.2 nm VolumePorod 100 nm3

SASDB66 – Deglycosylated myelin-associated glycoprotein full extracellular domain (Ig 1-5) N406Q mutant Sample: Myelin-associated glycoprotein (20-508; N406Q mutant) monomer, 54 kDa Mus musculus protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Jul 28 RgGuinier 7.8 nm Dmax 29.0 nm VolumePorod 216 nm3

SASDB76 – Deglycosylated myelin-associated glycoprotein (immunoglobulin domains 1-3) Sample: Myelin-associated glycoprotein Ig domains 1-3 monomer, 35 kDa Mus musculus protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11 RgGuinier 3.9 nm Dmax 12.6 nm VolumePorod 49 nm3