GRB2 dimerization mediated by SH2 domain-swapping is critical for T cell signaling and cytokine production.

Sandouk A, Xu Z, Baruah S, Tremblay M, Hopkins JB, Chakravarthy S, Gakhar L, Schnicker NJ, Houtman JCD
Sci Rep 13(1):3505 (2023 Mar 2)

PMID: 36864087 doi: 10.1038/s41598-023-30562-7

Submitted to SASBDB: 2022 Aug 18 Published in SASBDB: 2023 Mar 7

SASDP28 – Growth factor receptor-bound protein 2 (GRB2) - wild-type monomer Sample: Growth factor receptor-bound protein 2 monomer, 28 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31 RgGuinier 2.5 nm Dmax 9.0 nm VolumePorod 40 nm3

SASDP38 – Growth factor receptor-bound protein 2 (GRB2) - wild-type dimer Sample: Growth factor receptor-bound protein 2 dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31 RgGuinier 3.7 nm Dmax 14.0 nm VolumePorod 110 nm3

SASDP48 – Growth factor receptor-bound protein 2 (GRB2) - N188D/N214D mutant monomer Sample: Growth factor receptor-bound protein 2 (N188D, N214D) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31 RgGuinier 2.5 nm Dmax 9.5 nm VolumePorod 39 nm3

SASDP58 – Growth factor receptor-bound protein 2 (GRB2) - N188D/N214D mutant dimer Sample: Growth factor receptor-bound protein 2 (N188D, N214D) dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31 RgGuinier 4.1 nm Dmax 15.0 nm VolumePorod 140 nm3

SASDP68 – Growth factor receptor-bound protein 2 (GRB2) - V123D mutant monomer Sample: Growth factor receptor-bound protein 2 (V123D) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31 RgGuinier 2.6 nm Dmax 10.0 nm VolumePorod 45 nm3

SASDP78 – Growth factor receptor-bound protein 2 (GRB2) - V122P/V123P mutant dimer Sample: Growth factor receptor-bound protein 2 (V122P, V123P) dimer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31 RgGuinier 3.7 nm Dmax 20.4 nm VolumePorod 85 nm3