Structural analysis of the Candida albicans mitochondrial DNA maintenance factor Gcf1p reveals a dynamic DNA-bridging mechanism.

Tarrés-Solé A, Battistini F, Gerhold JM, Piétrement O, Martínez-García B, Ruiz-López E, Lyonnais S, Bernadó P, Roca J, Orozco M, Le Cam E, Sedman J, Solà M
Nucleic Acids Res (2023 May 19)
PMID: 37207342
doi: 10.1093/nar/gkad397
Submitted to SASBDB: 2022 Jul 11
Published in SASBDB:

SASDPY5 – Gcf1p protein

Gcf1p experimental SAS data
EOM/RANCH model
Sample: Gcf1p monomer, 26 kDa Candida albicans (strain … protein
Buffer: 50mM Tris, 750mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Oct 10
RgGuinier 3.7 nm
Dmax 14.5 nm
VolumePorod 80 nm3

SASDPZ5 – Gcf1p protein bound to DNA at 1.0 mg/mL

Gcf1pAf2_20 DNA experimental SAS data
WAXSIS model
Sample: Gcf1p monomer, 26 kDa Candida albicans (strain … protein
Af2_20 DNA monomer, 12 kDa DNA
Buffer: 25 mM Tris, 20 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30
RgGuinier 3.6 nm
Dmax 16.0 nm
VolumePorod 92 nm3

SASDP26 – Gcf1p protein bound to DNA at 2.0 mg/mL

Gcf1pAf2_20 DNA experimental SAS data
WAXSIS model
Sample: Gcf1p monomer, 26 kDa Candida albicans (strain … protein
Af2_20 DNA monomer, 12 kDa DNA
Buffer: 25 mM Tris, 20 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30
RgGuinier 3.9 nm
Dmax 16.0 nm
VolumePorod 133 nm3

SASDP36 – N-terminal truncated Gcf1p (amino acids 59-245) bound to DNA

Af2_20 DNAGcf1p(Δ58) experimental SAS data
OTHER model
Sample: Af2_20 DNA monomer, 12 kDa DNA
Gcf1p(Δ58) monomer, 22 kDa Candida albicans (strain … protein
Buffer: 25 mM Tris, 20 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 52 nm3