Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction.

Sülzen H, Began J, Dhillon A, Kereïche S, Pompach P, Votrubova J, Zahedifard F, Šubrtova A, Šafner M, Hubalek M, Thompson M, Zoltner M, Zoll S
Nat Commun 14(1):2403 (2023 Apr 27)

PMID: 37105991 doi: 10.1038/s41467-023-37988-7

Submitted to SASBDB: 2022 Aug 30 Published in SASBDB: 2023 Mar 27

SASDP99 – invariant surface glycoprotein ISG65 Sample: 65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein Buffer: 20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25 RgGuinier 3.3 nm Dmax 12.5 nm VolumePorod 53 nm3

SASDPA9 – invariant surface glycoprotein ISG65 bound to complement C3 (C3d) Sample: 65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein Complement C3 monomer, 35 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25 RgGuinier 3.5 nm Dmax 12.8 nm VolumePorod 104 nm3