Binding by calmodulin is coupled to transient unfolding of the third FF domain of Prp40A.

Díaz Casas A, Cordoba JJ, Ferrer BJ, Balakrishnan S, Wurm JE, Pastrana-Ríos B, Chazin WJ
Protein Sci 32(4):e4606 (2023 Apr)

PMID: 36810829 doi: 10.1002/pro.4606

Submitted to SASBDB: 2022 Aug 5 Published in SASBDB: 2023 Dec 7

SASDPV6 – Calmodulin Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein Buffer: 50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23 RgGuinier 2.2 nm Dmax 7.0 nm VolumePorod 38 nm3

SASDPW6 – FF3 domain of pre-mRNA-processing factor 40 homolog A (Prp40A) Sample: Pre-mRNA-processing factor 40 homolog A monomer, 9 kDa Homo sapiens protein Buffer: 50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23 RgGuinier 1.6 nm Dmax 7.2 nm VolumePorod 13 nm3

SASDPX6 – Calmodulin bound to the FF3 domain of pre-mRNA-processing factor 40 homolog A (Prp40A) Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein Pre-mRNA-processing factor 40 homolog A monomer, 9 kDa Homo sapiens protein Buffer: 50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23 RgGuinier 2.4 nm Dmax 8.7 nm VolumePorod 29 nm3