Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure

Miller R, Cummings C, Huang Q, Ando N, Gillilan R
Protein Science 31(12) (2022 Nov 17)

doi: 10.1002/pro.4489

Submitted to SASBDB: 2022 Jul 29 Published in SASBDB: 2022 Nov 24

SASDP47 – Glucose Isomerase at a neutral pH and 5 MPa (1 atm) of pressure Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein Buffer: 25 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7 RgGuinier 3.4 nm Dmax 10.6 nm VolumePorod 253 nm3

SASDP57 – Glucose Isomerase at a neutral pH and 100 MPa (1000 atm) of pressure Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein Buffer: 25 mM HEPES, 150 mM NaCl, and 3% v/v glycerol, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7 RgGuinier 3.5 nm Dmax 10.6 nm VolumePorod 267 nm3

SASDP67 – Bovine serum albumin at neutral pH, 4 °C, and 5 MPa (1 atm) of pressure Sample: Albumin monomer, 69 kDa Bos taurus protein Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7 RgGuinier 2.7 nm Dmax 8.5 nm VolumePorod 99 nm3

SASDP77 – Bovine serum albumin at neutral pH, 4 °C, and 100 MPa (1000 atm) of pressure Sample: Albumin monomer, 69 kDa Bos taurus protein Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7 RgGuinier 2.6 nm Dmax 8.3 nm VolumePorod 94 nm3

SASDP87 – Bovine serum albumin at neutral pH, 50 °C, and 5 MPa (1 atm) of pressure Sample: Albumin monomer, 69 kDa Bos taurus protein Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7 RgGuinier 3.0 nm Dmax 9.5 nm VolumePorod 108 nm3

SASDP97 – Bovine serum albumin at neutral pH, 50 °C, and 100 MPa (1000 atm) of pressure Sample: Albumin monomer, 69 kDa Bos taurus protein Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7 RgGuinier 2.8 nm Dmax 9.4 nm VolumePorod 104 nm3

SASDPA7 – Acidic leucine-rich nuclear phosphoprotein 32 family member A (pp32) at 4 °C and 5 MPa (1 atm) Sample: Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) monomer, 29 kDa Homo sapiens protein Buffer: 25 mM BisTRIS, 10 mM NaCl, 5 mM DTT, pH: 6.8 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7 RgGuinier 1.8 nm Dmax 6.0 nm VolumePorod 31 nm3

SASDPB7 – Acidic leucine-rich nuclear phosphoprotein 32 family member A (pp32) at 4 °C and 100 MPa (1000 atm) Sample: Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) monomer, 29 kDa Homo sapiens protein Buffer: 25 mM BisTRIS, 10 mM NaCl, 5 mM DTT, pH: 6.8 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7 RgGuinier 2.0 nm Dmax 7.5 nm VolumePorod 37 nm3