Tetracycline-modifying enzyme Sm TetX from Stenotrophomonas maltophilia

Malý M, Kolenko P, Stránský J, Švecová L, Dušková J, Koval' T, Skálová T, Trundová M, Adámková K, Černý J, Božíková P, Dohnálek J
Acta Crystallographica Section F Structural Biology Communications 79(7):180-192 (2023 Jul 05)

doi: 10.1107/S2053230X23005381 		Submitted to SASBDB: 2022 Aug 15
Published in SASBDB:

SASDPV7 – FAD-dependent monooxygenase from Stenotrophomonas maltophilia (no reducing agent)

Monooxygenase (M154I, A283T) experimental SAS data
REFMAC model
Sample: Monooxygenase (M154I, A283T) monomer, 39 kDa Stenotrophomonas maltophilia protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jan 10
RgGuinier 2.4 nm
Dmax 6.8 nm
VolumePorod 59 nm3

SASDPW7 – FAD-dependent monooxygenase from Stenotrophomonas maltophilia (with reducing agent)

Monooxygenase (M154I, A283T) experimental SAS data
REFMAC model
Sample: Monooxygenase (M154I, A283T) monomer, 39 kDa Stenotrophomonas maltophilia protein
Buffer: 25 mM Bis-Tris, 150 mM NaCl, 30 mM DTT, pH: 6.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jan 7
RgGuinier 2.4 nm
Dmax 6.4 nm
VolumePorod 64 nm3