Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY

Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

Submitted to SASBDB: 2022 Aug 15 Published in SASBDB: 2022 Sep 21

SASDPF7 – Ribonuclease A SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY) Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16 RgGuinier 1.5 nm Dmax 4.6 nm VolumePorod 16 nm3

SASDPG7 – Ribonuclease A SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY) Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8 RgGuinier 1.5 nm Dmax 4.8 nm VolumePorod 16 nm3

SASDPH7 – Urate Oxidase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY) Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15 RgGuinier 3.2 nm Dmax 8.8 nm VolumePorod 221 nm3

SASDPJ7 – Urate Oxidase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY) Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8 RgGuinier 3.2 nm Dmax 9.0 nm VolumePorod 214 nm3

SASDPK7 – Xylose isomerase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY) Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15 RgGuinier 3.2 nm Dmax 14.1 nm VolumePorod 228 nm3

SASDPL7 – Xylose isomerase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY) Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8 RgGuinier 3.3 nm Dmax 14.1 nm VolumePorod 233 nm3

SASDPM7 – Xylanase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY) Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16 RgGuinier 1.6 nm Dmax 4.8 nm VolumePorod 25 nm3

SASDPN7 – Xylanase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY) Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8 RgGuinier 1.6 nm Dmax 4.5 nm VolumePorod 24 nm3