Structure of the NPr:EINNtr Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems.

Strickland M, Stanley AM, Wang G, Botos I, Schwieters CD, Buchanan SK, Peterkofsky A, Tjandra N
Structure 24(12):2127-2137 (2016 Dec 6)
PMID: 27839951
doi: 10.1016/j.str.2016.10.007 		Submitted to SASBDB: 2016 Aug 29
Published in SASBDB:

SASDBZ5 – Enzyme I-Ntr (residues 170-424) in complex with NPr (residues 1-85)

Phosphocarrier protein NPrPhosphoenolpyruvate-protein phosphotransferase PtsP experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Phosphocarrier protein NPr monomer, 9 kDa Escherichia coli protein
Phosphoenolpyruvate-protein phosphotransferase PtsP monomer, 28 kDa Escherichia coli protein
Buffer: 10 mM Tris 150 mM NaCl 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, NIDDK, NIH on 2015 Jan 30
RgGuinier 2.3 nm
Dmax 8.2 nm
VolumePorod 52 nm3