Development of DNA Aptamers for Visualization of Glial Brain Tumors and Detection of Circulating Tumor Cells

Kichkailo A, Narodov A, Komarova M, Zamay T, Zamay G, Kolovskaya O, Erakhtin E, Glazyrin Y, Veprintsev D, Moryachkov R, Zabluda V, Shchugoreva I, Artyushenko P, Mironov V, Morozov D, Khorzhevskii V, Gorbushin A, Koshmanova A, Nikolaeva E, Grinev I, Voronkovskii I, Grek D, Belugin K, Volzhentsev A, Badmaev O, Luzan N, Lukyanenko K, Peters G, Lapin I, Kirichenko A, Konarev P, Morozov E, Mironov G, Gargaun A, Muharemagic D, Zamay S, Kochkina E, Dymova M, Smolyarova T, Sokolov A, Modestov A, Tokarev N, Shepelevich N, Ozerskaya A, Chanchikova N, Krat A, Zukov R, Bakhtina V, Shnyakin P, Shesternya P, Svetlichnyi V, Petrova M, Artyukhov I, Tomilin F, Berezovski M
Molecular Therapy - Nucleic Acids (2023 Mar)

doi: 10.1016/j.omtn.2023.03.015

Submitted to SASBDB: 2023 Mar 18 Published in SASBDB: 2023 Mar 30

SASDPZ8 – Gli-233 - ssDNA aptamer specific to glioma brain tumor cells Sample: Gli-233 - ssDNA aptamer to glioma brain tumor cells monomer, 10 kDa Artificially synthesized DNA Buffer: Dulbecco phosphate-buffered saline (DPBS, with Ca and Mg), pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 17 RgGuinier 1.9 nm Dmax 6.8 nm VolumePorod 13 nm3

SASDRY7 – Gli-55 - ssDNA aptamer to glioma brain tumor cells Sample: Gli-55 - ssDNA aptamer to glioma brain tumor cells monomer, 19 kDa synthetic construct DNA Buffer: Dulbecco phosphate-buffered saline (DPBS), pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Oct 30 RgGuinier 2.8 nm Dmax 11.5 nm VolumePorod 35 nm3

SASDP29 – Gli-55 - ssDNA aptamer specific to glioma brain tumor cells Sample: Gli-55 - ssDNA aptamer specific to glioma brain tumor cells monomer, 19 kDa Artificially synthesized DNA Buffer: Dulbecco phosphate-buffered saline (DPBS), pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Oct 30 RgGuinier 2.8 nm Dmax 11.5 nm VolumePorod 34 nm3