Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals.

Guerra JPL, Blanchet CE, Vieira BJC, Waerenborgh JC, Jones NC, Hoffmann SV, Pereira AS, Tavares P
Protein Sci :e4567 (2023 Jan 19)

PMID: 36658780 doi: 10.1002/pro.4567

Submitted to SASBDB: 2022 Sep 8 Published in SASBDB: 2023 Jan 26

SASDQT4 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.5 nm Dmax 20.0 nm VolumePorod 454 nm3

SASDQU4 – DNA-binding protein from starved cells: DgrDpsWT + 6 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.4 nm Dmax 18.4 nm VolumePorod 440 nm3

SASDQV4 – DNA-binding protein from starved cells: DgrDpsWT + 12 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.2 nm Dmax 15.5 nm VolumePorod 464 nm3

SASDQW4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.2 nm Dmax 14.1 nm VolumePorod 439 nm3

SASDQX4 – DNA-binding protein from starved cells: DgrDpsWT + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.2 nm Dmax 14.4 nm VolumePorod 427 nm3

SASDQY4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl, 5 mM EDTA Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS, 230 mM NaCl, 5 mM EDTA, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.5 nm Dmax 19.5 nm VolumePorod 453 nm3

SASDQZ4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.2 nm Dmax 13.9 nm VolumePorod 458 nm3

SASDQ25 – DNA-binding protein from starved cells: DgrDpsWT + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.4 nm Dmax 19.3 nm VolumePorod 438 nm3

SASDQ35 – DNA-binding protein from starved cells: DgrDps-D43A mutant in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.5 nm Dmax 19.8 nm VolumePorod 451 nm3

SASDQ45 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.4 nm Dmax 19.1 nm VolumePorod 412 nm3

SASDQ55 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.4 nm Dmax 20.7 nm VolumePorod 409 nm3

SASDQ65 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.5 nm Dmax 20.6 nm VolumePorod 404 nm3

SASDQ75 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8 RgGuinier 4.5 nm Dmax 19.5 nm VolumePorod 431 nm3