Probing the conformational changes of in vivo overexpressed cell cycle regulator 6S ncRNA

Makraki E, Miliara S, Pagkalos M, Kokkinidis M, Mylonas E, Fadouloglou V
Frontiers in Molecular Biosciences 10 (2023 Jul 17)

doi: 10.3389/fmolb.2023.1219668 		Submitted to SASBDB: 2022 Sep 8
Published in SASBDB:

SASDQR4 – Escherichia coli 6S RNA

6S RNA (SsrS gene) experimental SAS data
Escherichia coli 6S RNA Rg histogram
Sample: 6S RNA (SsrS gene) monomer, 59 kDa Escherichia coli RNA
Buffer: 20 mM Tris-HCl, 200 mM KCl, 5 mM MgCl2, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 4
RgGuinier 6.1 nm
Dmax 24.0 nm
VolumePorod 175 nm3

SASDQS4 – Escherichia coli 6S:pRNA complex

6S RNA (SsrS gene)product RNA from E. coli 6S experimental SAS data
Escherichia coli 6S:pRNA complex Rg histogram
Sample: 6S RNA (SsrS gene) monomer, 59 kDa Escherichia coli RNA
Product RNA from E. coli 6S monomer, 7 kDa Escherichia coli RNA
Buffer: 20 mM Tris-HCl, 200 mM KCl, 5 mM MgCl2, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 4
RgGuinier 4.9 nm
Dmax 20.0 nm
VolumePorod 260 nm3