Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase.

Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
Cell Rep 42(8):112972 (2023 Aug 13)
PMID: 37578860
doi: 10.1016/j.celrep.2023.112972 		Submitted to SASBDB: 2022 Sep 16
Published in SASBDB:

SASDQ95 – The complex formed between E. coli S-adenosylmethionine synthase and wild-type S-adenosyl-L-methionine lyase from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Jun 17
RgGuinier 4.8 nm
Dmax 17.5 nm
VolumePorod 325 nm3

SASDQA5 – The complex formed between E. coli S-adenosylmethionine synthase and S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase (E68Q/Q94A) experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase (E68Q/Q94A) tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 May 7
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 326 nm3