Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA.

Keil P, Wulf A, Kachariya N, Reuscher S, Hühn K, Silbern I, Altmüller J, Keller M, Stehle R, Zarnack K, Sattler M, Urlaub H, Sträßer K
Nucleic Acids Res (2022 Dec 30)

PMID: 36583366 doi: 10.1093/nar/gkac1206

Submitted to SASBDB: 2022 Sep 21 Published in SASBDB: 2023 Jan 6

SASDQT5 – NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) wild-type Sample: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein Buffer: 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5 Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20 RgGuinier 2.0 nm Dmax 6.8 nm VolumePorod 24 nm3

SASDQU5 – NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) P196D/A197D linker mutant Sample: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein Buffer: 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5 Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20 RgGuinier 1.9 nm Dmax 6.7 nm VolumePorod 25 nm3

SASDQV5 – NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) wild-type, bound to RNA Sample: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein Short RNA oligonucleotide (NPL3 binding sequence) monomer, 4 kDa RNA Buffer: 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5 Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20 RgGuinier 2.0 nm Dmax 6.3 nm VolumePorod 30 nm3