Transient interdomain interactions in free USP14 shape its conformational ensemble.

Salomonsson J, Wallner B, Sjöstrand L, D'Arcy P, Sunnerhagen M, Ahlner A
Protein Sci 33(5):e4975 (2024 May)
PMID: 38588275
doi: 10.1002/pro.4975 		Submitted to SASBDB: 2022 Nov 2
Published in SASBDB:

SASDQZ7 – Ubiquitin-specific protease 14 (USP14)

Ubiquitin carboxyl-terminal hydrolase 14 experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Ubiquitin carboxyl-terminal hydrolase 14 monomer, 56 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Oct 18
RgGuinier 3.1 nm
Dmax 12.5 nm
VolumePorod 111 nm3

SASDQ28 – Catalytic domain of ubiquitin-specific protease 14 (USP14)

Ubiquitin carboxyl-terminal hydrolase 14 experimental SAS data
DAMMIN model
Sample: Ubiquitin carboxyl-terminal hydrolase 14 monomer, 46 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 5
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 88 nm3