Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes.

Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
Protein Sci :e4564 (2023 Jan 6)

PMID: 36606712 doi: 10.1002/pro.4564

Submitted to SASBDB: 2022 Oct 24 Published in SASBDB: 2023 Jan 10

SASDQ47 – N-terminal GST-like domain of Plasmodium berghei tRNA import protein Sample: TRNA import protein tRIP dimer, 49 kDa Plasmodium berghei (strain … protein Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7 Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9 RgGuinier 2.9 nm Dmax 11.0 nm VolumePorod 68 nm3

SASDQ37 – N-terminal GST-like domain of Plasmodium berghei cytosolic glutamyl-tRNA synthetase Sample: Glutamate--tRNA ligase dimer, 57 kDa Plasmodium berghei (strain … protein Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7 Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9 RgGuinier 3.0 nm Dmax 11.5 nm VolumePorod 96 nm3

SASDQ57 – The scaffold of Plasmodium berghei multi aminoacyl-tRNA synthetase complexes Sample: Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase dimer, 105 kDa Plasmodium berghei ANKA protein Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7 Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9 RgGuinier 3.9 nm Dmax 14.1 nm VolumePorod 145 nm3

SASDQ67 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, Q-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and glutaminyl-tRNA synthetase Sample: Glutamine--tRNA ligase dimer, 46 kDa Plasmodium berghei (strain … protein Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein TRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7 Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9 RgGuinier 4.6 nm Dmax 17.1 nm VolumePorod 198 nm3

SASDQ77 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, M-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and methionyl-tRNA synthetase Sample: Methionine--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein TRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7 Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9 RgGuinier 4.7 nm Dmax 18.3 nm VolumePorod 217 nm3