Circularized and solubility‐enhanced MSP s facilitate simple and high‐yield production of stable nanodiscs for studies of membrane proteins in solution

Johansen N, Tidemand F, Nguyen T, Rand K, Pedersen M, Arleth L
The FEBS Journal 286(9):1734-1751 (2019 Feb 12)

doi: 10.1111/febs.14766 		Submitted to SASBDB: 2022 Oct 13
Published in SASBDB:

SASDQP6 – Nanodisc with POPC and circularized membrane scaffolding protein (csMSP1E3D1)

circularized Membrane scaffolding protein 1 E3 D11-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Circularized Membrane scaffolding protein 1 E3 D1, 62 kDa protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) None, lipid
Buffer: 20 mM Tris-HCl pH 7.5, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 5
RgGuinier 5.8 nm
Dmax 14.5 nm