eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation.

Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
Nucleic Acids Res (2023 Feb 2)

PMID: 36727461 doi: 10.1093/nar/gkad030

Submitted to SASBDB: 2022 Oct 17 Published in SASBDB: 2023 Jan 4

SASDQT6 – apo-form of eukaryotic initiation factor 4A1 Sample: Eukaryotic initiation factor 4A-I monomer, 46 kDa Homo sapiens protein Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 22 RgGuinier 3.1 nm Dmax 9.8 nm VolumePorod 80 nm3

SASDQU6 – eukaryotic initiation factor 4A1 bound to 20 nt (AG)10 ssRNA Sample: Eukaryotic initiation factor 4A-I monomer, 46 kDa Homo sapiens protein (AG)10-RNA monomer, 7 kDa synthetic construct RNA Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 16 RgGuinier 2.8 nm Dmax 10.5 nm VolumePorod 96 nm3

SASDQV6 – eukaryotic initiation factor 4A1 bound to 20 nt (CAA)6CA ssRNA Sample: Eukaryotic initiation factor 4A-I monomer, 46 kDa Homo sapiens protein (CAA)6CA-RNA monomer, 6 kDa synthetic construct RNA Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 22 RgGuinier 3.1 nm Dmax 12.8 nm VolumePorod 89 nm3

SASDQW6 – multimeric eukaryotic initiation factor 4A1 bound to 20 nt (AG)10 ssRNA Sample: (AG)10-RNA monomer, 7 kDa synthetic construct RNA Eukaryotic initiation factor 4A-I trimer, 138 kDa Homo sapiens protein Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 16 RgGuinier 3.4 nm Dmax 13.1 nm VolumePorod 150 nm3

SASDQX6 – multimeric eukaryotic initiation factor 4A1 bound to 20 nt (AG)10-24bp duplex RNA Sample: Eukaryotic initiation factor 4A-I trimer, 138 kDa Homo sapiens protein (AG)10-24bp loading-RNA monomer, 15 kDa synthetic construct RNA (AG)10-24bp duplex-RNA monomer, 7 kDa synthetic construct RNA Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 16 RgGuinier 4.9 nm Dmax 16.2 nm VolumePorod 230 nm3