Architecture of a PKS-NRPS hybrid megaenzyme involved in the biosynthesis of the genotoxin colibactin

Bonhomme S, Contreras-Martel C, Dessen A, Macheboeuf P
Structure (2023 Apr)

doi: 10.1016/j.str.2023.03.012 		Submitted to SASBDB: 2022 Oct 18
Published in SASBDB:

SASDQ87 – Solution structure of colibactin ClbK

Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK experimental SAS data
Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK Kratky plot
Sample: Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK dimer, 476 kDa Escherichia coli protein
Buffer: 50 mM Hepes pH 7.5, 200 mM NaCl, 5 mM DTT, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Sep 14
RgGuinier 8.0 nm
Dmax 30.0 nm
VolumePorod 1340 nm3