Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1

Vish K, Stiegler A, Boggon T
Journal of Biological Chemistry :105098 (2023 Jul)

doi: 10.1016/j.jbc.2023.105098

Submitted to SASBDB: 2023 Mar 2 Published in SASBDB: 2023 Jul 27

SASDRJ6 – SH2-SH3-SH2 domains of Ras GTPase-activating protein 1 (p120RasGAP) Sample: Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8, 350 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 12 RgGuinier 2.5 nm Dmax 9.8 nm VolumePorod 42 nm3

SASDRK6 – SH2-SH3-SH2 domains of Ras GTPase-activating protein 1 (p120RasGAP) bound to a doubly phosphorylated EphB4 peptide Sample: Ephrin type-B receptor 4 monomer, 2 kDa Homo sapiens protein Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8, 350 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 12 RgGuinier 2.7 nm Dmax 10.5 nm VolumePorod 44 nm3

SASDRL6 – SH2-SH3-SH2 domains of Ras GTPase-activating protein 1 (p120RasGAP) bound to a doubly phosphorylated p190RhoGAP peptide Sample: Rho GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8, 350 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 12 RgGuinier 2.4 nm Dmax 7.7 nm VolumePorod 44 nm3

SASDRE6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 4 RgGuinier 4.0 nm Dmax 14.0 nm VolumePorod 172 nm3

SASDRF6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to doubly phosphorylated EphB4 peptide Sample: Ephrin type-B receptor 4 monomer, 2 kDa Homo sapiens protein Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 4 RgGuinier 3.9 nm Dmax 13.7 nm VolumePorod 176 nm3

SASDRG6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to doubly phosphorylated p190RhoGAP peptide Sample: Rho GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 4 RgGuinier 4.0 nm Dmax 15.1 nm VolumePorod 194 nm3

SASDRH6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to a doubly phosphorylated Dok1 peptide Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein Docking protein 1 monomer, 3 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 4 RgGuinier 4.0 nm Dmax 14.6 nm VolumePorod 196 nm3