Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies

Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S
International Journal of Molecular Sciences 24(1):399 (2022 Dec 26)

doi: 10.3390/ijms24010399

Submitted to SASBDB: 2022 Nov 1 Published in SASBDB: 2022 Dec 30

SASDQB7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region) Sample: Non-structural protein V monomer, 15 kDa Hendra virus (isolate … protein Buffer: 50 mM sodium phosphate, pH: 7.2 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14 RgGuinier 3.7 nm Dmax 19.0 nm VolumePorod 48 nm3

SASDQC7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region) mutated YYY --> AAA Sample: Non-structural protein V (Y211A, Y212A, Y213A mutant) monomer, 15 kDa Hendra virus (isolate … protein Buffer: 50 mM sodium phosphate, pH: 7.2 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14 RgGuinier 4.0 nm Dmax 19.0 nm VolumePorod 49 nm3

SASDQD7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region, C-terminal truncated) Sample: Non-structural protein V monomer, 9 kDa Hendra virus (isolate … protein Buffer: 50 mM sodium phosphate, pH: 7.2 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14 RgGuinier 2.8 nm Dmax 13.0 nm VolumePorod 22 nm3

SASDQE7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region C-terminal truncated), mutated YYY --> AAA Sample: Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) monomer, 9 kDa Hendra virus (isolate … protein Buffer: 50 mM sodium phosphate, pH: 7.2 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14 RgGuinier 2.7 nm Dmax 12.5 nm VolumePorod 21 nm3

SASDQF7 – Region within N-terminal domain of P/V/W protein of Nipah virus (PNT3 region) Sample: Non-structural protein V monomer, 15 kDa Nipah henipavirus protein Buffer: 50 mM sodium phosphate, pH: 7.2 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14 RgGuinier 3.7 nm Dmax 16.5 nm VolumePorod 47 nm3