Visualizing RNA conformational and architectural heterogeneity in solution.
Ding J,
Lee YT,
Bhandari Y,
Schwieters CD,
Fan L,
Yu P,
Tarosov SG,
Stagno JR,
Ma B,
Nussinov R,
Rein A,
Zhang J,
Wang YX
Nat Commun
14(1):714
(2023 Feb 9)
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Sample: |
Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain, 68 kDa marine metagenome RNA
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Buffer: |
50 mM MES pH 6.0, 10 mM KCl, 1 mM MgCl2, pH: 6 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Apr 18
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|
RgGuinier |
5.9 |
nm |
Dmax |
20.7 |
nm |
VolumePorod |
206 |
nm3 |
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|
Sample: |
Thermoanearobacter tengcongensis (Tte) fecB riboswitch aptamer domain, 68 kDa marine metagenome RNA
|
Buffer: |
50 mM MES pH 6.0, 10 mM KCl, 1 mM MgCl2, 4-18 μM coenzyme B12 ligand, pH: 6 |
Experiment: |
SAXS
data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Apr 18
|
|
RgGuinier |
5.7 |
nm |
Dmax |
19.7 |
nm |
VolumePorod |
230 |
nm3 |
|
|