A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis.
Pierson E,
De Pol F,
Fillet M,
Wouters J
Commun Biol
6(1):1024
(2023 Oct 10)
|
|
|
Sample: |
Phosphoserine phosphatase SerB2 dimer, 87 kDa Mycobacterium tuberculosis (strain … protein
|
Buffer: |
50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4 |
Experiment: |
SAXS
data collected at SWING, SOLEIL on 2021 Oct 6
|
|
RgGuinier |
3.2 |
nm |
Dmax |
10.7 |
nm |
VolumePorod |
141 |
nm3 |
|
|
|
|
|
Sample: |
Mycobacterium tuberculosis phosphoserine phosphatase SerB2 tetramer, 173 kDa Mycobacterium tuberculosis (strain … protein
|
Buffer: |
50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4 |
Experiment: |
SAXS
data collected at SWING, SOLEIL on 2021 Oct 6
|
|
RgGuinier |
4.0 |
nm |
Dmax |
12.5 |
nm |
VolumePorod |
307 |
nm3 |
|
|
|
|
|
Sample: |
Phosphoserine phosphatase SerB2 trimer, 130 kDa Mycobacterium tuberculosis (strain … protein
|
Buffer: |
50 mM Tris, 150 mM NaCl, 1 mM TCEP, 10 mM L-serine, pH: 7.4 |
Experiment: |
SAXS
data collected at SWING, SOLEIL on 2021 Oct 6
|
|
RgGuinier |
4.0 |
nm |
Dmax |
14.3 |
nm |
VolumePorod |
200 |
nm3 |
|
|
|
|
|
Sample: |
Phosphoserine phosphatase dimer, 88 kDa Mycobacterium marinum (strain … protein
|
Buffer: |
50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4 |
Experiment: |
SAXS
data collected at SWING, SOLEIL on 2021 Oct 6
|
|
RgGuinier |
3.2 |
nm |
Dmax |
9.9 |
nm |
VolumePorod |
138 |
nm3 |
|
|
|
|
|
Sample: |
Phosphoserine phosphatase (G31R, G152E) dimer, 88 kDa Mycobacterium avium (strain … protein
|
Buffer: |
50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4 |
Experiment: |
SAXS
data collected at SWING, SOLEIL on 2021 Oct 6
|
|
RgGuinier |
3.3 |
nm |
Dmax |
10.1 |
nm |
VolumePorod |
138 |
nm3 |
|
|