A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis.

Pierson E, De Pol F, Fillet M, Wouters J
Commun Biol 6(1):1024 (2023 Oct 10)
PMID: 37817000
doi: 10.1038/s42003-023-05402-z
Submitted to SASBDB: 2023 Jan 24
Published in SASBDB:

SASDRS4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) dimer

Phosphoserine phosphatase SerB2 experimental SAS data
Phosphoserine phosphatase SerB2 Kratky plot
Sample: Phosphoserine phosphatase SerB2 dimer, 87 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 141 nm3

SASDRT4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) tetramer

Mycobacterium tuberculosis phosphoserine phosphatase SerB2 experimental SAS data
DADIMODO model
Sample: Mycobacterium tuberculosis phosphoserine phosphatase SerB2 tetramer, 173 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
RgGuinier 4.0 nm
Dmax 12.5 nm
VolumePorod 307 nm3

SASDRU4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) trimer formed in the presence of L-serine

Phosphoserine phosphatase SerB2 experimental SAS data
Phosphoserine phosphatase SerB2 Kratky plot
Sample: Phosphoserine phosphatase SerB2 trimer, 130 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, 10 mM L-serine, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
RgGuinier 4.0 nm
Dmax 14.3 nm
VolumePorod 200 nm3

SASDRV4 – Mycobacterium marinum phosphoserine phosphatase (MmSerB2) dimer

phosphoserine phosphatase experimental SAS data
phosphoserine phosphatase Kratky plot
Sample: Phosphoserine phosphatase dimer, 88 kDa Mycobacterium marinum (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
RgGuinier 3.2 nm
Dmax 9.9 nm
VolumePorod 138 nm3

SASDRW4 – Mycobacterium avium phosphoserine phosphatase (MaSerB) dimer

Phosphoserine phosphatase (G31R, G152E) experimental SAS data
DADIMODO model
Sample: Phosphoserine phosphatase (G31R, G152E) dimer, 88 kDa Mycobacterium avium (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
RgGuinier 3.3 nm
Dmax 10.1 nm
VolumePorod 138 nm3