Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity

Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
Nature Communications 14(1) (2023 Jul 13)

doi: 10.1038/s41467-023-39808-4

Submitted to SASBDB: 2022 Nov 27 Published in SASBDB: 2023 May 18

SASDQE8 – G. gallus TRPV4 Ankyrin Repeat Domain Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 28 kDa Gallus gallus protein Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16 RgGuinier 2.3 nm Dmax 11.5 nm VolumePorod 41 nm3

SASDQF8 – G. gallus TRPV4 N-terminal Domain Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 43 kDa Gallus gallus protein Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16 RgGuinier 3.7 nm Dmax 19.0 nm VolumePorod 59 nm3

SASDQG8 – G. gallus TRPV4 N-terminal Domain, mutant 107-KRWRR-111 to 107-AAWAA-111 Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 42 kDa Gallus gallus protein Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16 RgGuinier 4.0 nm Dmax 19.5 nm VolumePorod 78 nm3

SASDQH8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N54 Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 37 kDa Gallus gallus protein Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16 RgGuinier 2.6 nm Dmax 9.5 nm VolumePorod 64 nm3

SASDQJ8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N97 Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 33 kDa Gallus gallus protein Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16 RgGuinier 2.5 nm Dmax 11.9 nm VolumePorod 41 nm3

SASDQK8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N104 Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 32 kDa Gallus gallus protein Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16 RgGuinier 2.8 nm Dmax 13.0 nm VolumePorod 49 nm3

SASDQL8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N120 Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 29 kDa Gallus gallus protein Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16 RgGuinier 2.7 nm Dmax 12.5 nm VolumePorod 43 nm3

SASDQM8 – G. gallus TRPV4 intrinsically disordered N-terminus Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 15 kDa Gallus gallus protein Buffer: 20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16 RgGuinier 3.2 nm Dmax 14.5 nm VolumePorod 59 nm3

SASDQN8 – G. gallus TRPV4 intrinsically disordered N-terminus, mutation 107-KRWRR-111 to 107-AAWAA-111 Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 14 kDa Gallus gallus protein Buffer: 20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16 RgGuinier 3.3 nm Dmax 14.5 nm VolumePorod 37 nm3