Identification and characterization of endo-α-, exo-α-, and exo-β-d-arabinofuranosidases degrading lipoarabinomannan and arabinogalactan of mycobacteria

Shimokawa M, Ishiwata A, Kashima T, Nakashima C, Li J, Fukushima R, Sawai N, Nakamori M, Tanaka Y, Kudo A, Morikami S, Iwanaga N, Akai G, Shimizu N, Arakawa T, Yamada C, Kitahara K, Tanaka K, Ito Y, Fushinobu S, Fujita K
Nature Communications 14(1) (2023 Sep 19)

doi: 10.1038/s41467-023-41431-2 		Submitted to SASBDB: 2022 Nov 29
Published in SASBDB:

SASDQQ8 – Endo-D-arabinanase, 54 kDa, dimer, Microbacterium arabinogalactanolyticum

Endo-D-arabinanase experimental SAS data
REFMAC model
Sample: Endo-D-arabinanase dimer, 107 kDa Microbacterium arabinogalactanolyticum protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2022 Nov 3
RgGuinier 3.1 nm
Dmax 10.4 nm
VolumePorod 131 nm3