SAXS characterisation of CALX1.2 CBD12 construct

Roberto Kopke Salinas Submitted to SASBDB: 2022 Dec 11
Published in SASBDB:

SASDQV8 – Na/Ca-exchange protein - CALX1.2 CBD12 domain in the Ca2+-free (Apo) state

Na/Ca-exchange protein, isoform D experimental SAS data
Na/Ca-exchange protein, isoform D Kratky plot
Sample: Na/Ca-exchange protein, isoform D monomer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM TRIS, 200 mM NaCl, 1% v/v glycerol, 0.03% w/v NaN3, 1 mM β-mercaptoethanol, 2 mM EDTA, pH: 7.5
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 48 nm3

SASDQW8 – Na/Ca-exchange protein - CALX1.2 CBD12 domain in the Ca2+-bound state

Na/Ca-exchange protein, isoform D experimental SAS data
Na/Ca-exchange protein, isoform D Kratky plot
Sample: Na/Ca-exchange protein, isoform D monomer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM TRIS, 200 mM NaCl, 1% v/v glycerol, 0.03% w/v NaN3, 1 mM β-mercaptoethanol, 50 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8
RgGuinier 2.7 nm
Dmax 9.9 nm
VolumePorod 32 nm3