7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system.

Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D
Nucleic Acids Res (2023 Mar 17)

PMID: 36928176 doi: 10.1093/nar/gkad141

Submitted to SASBDB: 2022 Dec 24 Published in SASBDB: 2023 Feb 20

SASDQ59 – Mutant 7-deazapurine in DNA protein A (D95A) Sample: DNA-guanine transglycosylase - D95A mutant monomer, 50 kDa Salmonella enterica subsp. … protein Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22 RgGuinier 2.7 nm Dmax 10.1 nm VolumePorod 67 nm3

SASDQ89 – 7-deazapurine in DNA protein B Sample: DGQHR domain-containing protein dimer, 88 kDa Salmonella enterica subsp. … protein Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22 RgGuinier 3.4 nm Dmax 13.5 nm VolumePorod 126 nm3

SASDQ79 – Mutant 7-deazapurine in DNA protein A (D95A) bound to a 28 base pair DNA substrate (2-state model: complex plus free DNA) Sample: Custom 28 base pair double stranded DNA monomer, 17 kDa Synthetic, purchased from … DNA DNA-guanine transglycosylase - D95A mutant monomer, 50 kDa Salmonella enterica subsp. … protein Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22 RgGuinier 3.4 nm Dmax 13.5 nm VolumePorod 104 nm3

SASDQ69 – 28 base pair DNA substrate (used in experiments with 7-deazapurine in DNA proteins) Sample: Custom 28 base pair double stranded DNA monomer, 17 kDa Synthetic, purchased from … DNA Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22 RgGuinier 2.6 nm Dmax 10.2 nm VolumePorod 24 nm3