Investigating RNA-RNA interactions through computational and biophysical analysis.

Mrozowich T, Park SM, Waldl M, Henrickson A, Tersteeg S, Nelson CR, De Klerk A, Demeler B, Hofacker IL, Wolfinger MT, Patel TR
Nucleic Acids Res (2023 Mar 31)

PMID: 36999609 doi: 10.1093/nar/gkad223

Submitted to SASBDB: 2022 Dec 30 Published in SASBDB: 2023 Apr 11

SASDQL9 – Japanese encephalitis virus 5' TR RNA Sample: Japanese encephaltitis 5' TR monomer, 74 kDa Japanese encephalitis virus RNA Buffer: 10 mM Bis-tris, 100 mM NaCl, 15 mM KCl 15 mM MgCl2, 10% glycerol, pH: 5 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 22 RgGuinier 7.0 nm Dmax 22.4 nm VolumePorod 356 nm3

SASDQM9 – Japanese encephalitis virus 3' UTR RNA Sample: Japanese encephalitis virus 3' UTR monomer, 186 kDa Japanese encephalitis virus RNA Buffer: 10 mM Bis-tris, 100 mM NaCl, 15 mM KCl 15 mM MgCl2, 10% glycerol, pH: 5 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 22 RgGuinier 11.3 nm Dmax 34.6 nm VolumePorod 2900 nm3

SASDQN9 – Japanese encephalitis virus 5' TR and 3' UTR RNA complex Sample: Japanese encephalitis virus 5' TR and 3' UTR complex monomer, 260 kDa Japanese encephalitis virus RNA Buffer: 10 mM Bis-tris, 100 mM NaCl, 15 mM KCl 15 mM MgCl2, 10% glycerol, pH: 5 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 22 RgGuinier 12.8 nm Dmax 40.0 nm VolumePorod 4550 nm3