Structural insights into the formation of repulsive netrin guidance complexes.

Priest JM, Nichols EL, Smock RG, Hopkins JB, Mendoza JL, Meijers R, Shen K, Özkan E
Sci Adv 10(7):eadj8083 (2024 Feb 16)

PMID: 38363837 doi: 10.1126/sciadv.adj8083

Submitted to SASBDB: 2022 Dec 26 Published in SASBDB: 2023 Jan 2

SASDQJ9 – An assembly between C-terminal truncated Netrin UNC-6 and the extracellular domain of Netrin receptor UNC-5 with porcine intestinal mucosa heparin (UNC-6ΔC-heparin-UNC-5 ECD complex) Sample: Netrin unc-6 monomer, 52 kDa Caenorhabditis elegans protein Isoform a of Netrin receptor unc-5 monomer, 38 kDa Caenorhabditis elegans protein Heparin, porcine intestinal mucosa monomer, 15 kDa Sus scrofa domesticus Buffer: 10 mM HEPES pH 7.2, 150 mM NaCl, 100 mM MgSO4, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 18 RgGuinier 10.4 nm Dmax 37.3 nm VolumePorod 2156 nm3

SASDQK9 – An assembly between C-terminal truncated Netrin UNC-6, the extracellular domains of Netrin receptor UNC-5 and UNC-40 with porcine intestinal mucosa heparin (UNC-6ΔC-heparin-UNC-5 ECD-UNC-40 ECD complex) Sample: Netrin unc-6 monomer, 52 kDa Caenorhabditis elegans protein Isoform a of Netrin receptor unc-5 monomer, 38 kDa Caenorhabditis elegans protein Heparin, porcine intestinal mucosa monomer, 15 kDa Sus scrofa domesticus Netrin receptor unc-40 monomer, 118 kDa Caenorhabditis elegans protein Buffer: 10 mM HEPES pH 7.2, 150 mM NaCl, 100 mM MgSO4, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Dec 11 RgGuinier 10.6 nm Dmax 36.5 nm VolumePorod 3410 nm3