Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis.

Wang H, Xie C, Deng B, Ding J, Li N, Kou Z, Jin M, He J, Wang Q, Wen H, Zhang J, Zhou Q, Chen S, Chen X, Yuan TF, Zhu S
Nat Struct Mol Biol (2024 Sep 3)

PMID: 39227720 doi: 10.1038/s41594-024-01387-3

Submitted to SASBDB: 2023 Jan 10 Published in SASBDB: 2024 Sep 5

SASDR32 – Human derived autoantibody mAb2G7 at pH 8.0 Sample: Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein Buffer: phosphate buffered saline, pH: 8 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8 RgGuinier 5.0 nm Dmax 16.0 nm VolumePorod 260 nm3

SASDR42 – Human derived autoantibody mAb5F6 at pH 8.0 Sample: Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein Buffer: phosphate buffered saline, pH: 8 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8 RgGuinier 5.0 nm Dmax 15.8 nm VolumePorod 252 nm3

SASDR52 – Human GluN1-GluN2A NMDA receptor at pH 8.0 Sample: Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein Buffer: 150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8 RgGuinier 6.6 nm Dmax 20.4 nm VolumePorod 1180 nm3

SASDR62 – Human GluN1-GluN2A NMDA receptor in complex with human derived autoantibody mAb2G7 at pH 8.0 Sample: Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein Buffer: 150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8 RgGuinier 7.7 nm Dmax 25.4 nm VolumePorod 1260 nm3

SASDR72 – Human GluN1-GluN2A NMDA receptor in complex with human derived autoantibody mAb5F6 at pH 8.0 Sample: Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein Human derived autoantibody mAb5F6 heavy chain, mAb5F6 VH dimer, 104 kDa Homo sapiens protein Human derived autoantibody mAb5F6 light chain, mAb5F6 VL dimer, 52 kDa protein Buffer: 150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8 RgGuinier 9.9 nm Dmax 31.7 nm VolumePorod 2500 nm3