Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis.
Wang H,
Xie C,
Deng B,
Ding J,
Li N,
Kou Z,
Jin M,
He J,
Wang Q,
Wen H,
Zhang J,
Zhou Q,
Chen S,
Chen X,
Yuan TF,
Zhu S
Nat Struct Mol Biol
(2024 Sep 3)
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Sample: |
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein
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Buffer: |
phosphate buffered saline, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
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RgGuinier |
5.0 |
nm |
Dmax |
16.0 |
nm |
VolumePorod |
260 |
nm3 |
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Sample: |
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein
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Buffer: |
phosphate buffered saline, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
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RgGuinier |
5.0 |
nm |
Dmax |
15.8 |
nm |
VolumePorod |
252 |
nm3 |
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Sample: |
Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein
Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein
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Buffer: |
150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
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RgGuinier |
6.6 |
nm |
Dmax |
20.4 |
nm |
VolumePorod |
1180 |
nm3 |
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Sample: |
Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein
Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein
|
Buffer: |
150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
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RgGuinier |
7.7 |
nm |
Dmax |
25.4 |
nm |
VolumePorod |
1260 |
nm3 |
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Sample: |
Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein
Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein
Human derived autoantibody mAb5F6 heavy chain, mAb5F6 VH dimer, 104 kDa Homo sapiens protein
Human derived autoantibody mAb5F6 light chain, mAb5F6 VL dimer, 52 kDa protein
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Buffer: |
150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8 |
Experiment: |
SAXS
data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
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RgGuinier |
9.9 |
nm |
Dmax |
31.7 |
nm |
VolumePorod |
2500 |
nm3 |
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