A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria.

Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
Nat Commun 15(1):3167 (2024 Apr 12)

PMID: 38609367 doi: 10.1038/s41467-024-47486-z

Submitted to SASBDB: 2023 Feb 4 Published in SASBDB: 2024 Mar 4

SASDRH5 – A ssr1698 Dri1 hemoprotein, wild-type (as-purified) variant Sample: Ssr1698 protein monomer, 11 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2021 Dec 12 RgGuinier 1.6 nm Dmax 5.3 nm VolumePorod 12 nm3

SASDRJ5 – A ssr1698 Dri1 hemoprotein, wild-type (as-purified) variant + heme Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 13 RgGuinier 1.9 nm Dmax 6.2 nm VolumePorod 26 nm3

SASDRG5 – A ssr1698 Dri1 hemoprotein, wild-type (apo) variant + heme Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27 RgGuinier 2.0 nm Dmax 6.3 nm VolumePorod 29 nm3

SASDRK5 – A ssr1698 Dri1 hemoprotein, wild-type (Zn2+) variant Sample: Ssr1698 protein monomer, 11 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27 RgGuinier 1.6 nm Dmax 5.0 nm VolumePorod 10 nm3

SASDRL5 – A ssr1698 Dri1 hemoprotein, wild-type (Zn2+) variant + heme Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27 RgGuinier 2.0 nm Dmax 6.2 nm VolumePorod 29 nm3

SASDRM5 – A ssr1698 Dri1 hemoprotein, wild-type (Co2+) variant + heme Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 13 RgGuinier 2.0 nm Dmax 6.3 nm VolumePorod 29 nm3

SASDRF5 – A ssr1698 Dri1 hemoprotein, H21A variant Sample: Ssr1698 protein (H21A) monomer, 11 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 10 RgGuinier 1.6 nm Dmax 5.7 nm VolumePorod 12 nm3

SASDQS9 – A ssr1698 Dri1 hemoprotein, H21A variant + heme Sample: Ssr1698 protein (H21A) dimer, 22 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Dec 7 RgGuinier 2.0 nm Dmax 6.5 nm VolumePorod 28 nm3

SASDRE5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant Sample: Ssr1698 protein (H79A:R90A) monomer, 11 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Mar 30 RgGuinier 1.6 nm Dmax 5.5 nm VolumePorod 12 nm3

SASDRD5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant + heme Sample: Ssr1698 protein (H79A:R90A) dimer, 22 kDa Synechocystis sp. (strain … protein Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Mar 30 RgGuinier 2.0 nm Dmax 6.3 nm VolumePorod 28 nm3