Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum

Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
Structure (2023 Mar)

doi: 10.1016/j.str.2023.03.002

Submitted to SASBDB: 2023 Feb 25 Published in SASBDB: 2023 Mar 27

SASDRX5 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc1 Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein Synthetic antigen binding fragment tc1 monomer, 48 kDa synthetic construct protein Buffer: phosphate buffered saline, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23 RgGuinier 3.8 nm Dmax 14.0 nm VolumePorod 125 nm3

SASDRY5 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc2 Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein Synthetic antigen binding fragment tc2 monomer, 48 kDa synthetic construct protein Buffer: phosphate buffered saline, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23 RgGuinier 3.8 nm Dmax 14.0 nm VolumePorod 125 nm3

SASDRZ5 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc3 Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein Synthetic antigen binding fragment tc3 monomer, 49 kDa synthetic construct protein Buffer: phosphate buffered saline, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23 RgGuinier 3.6 nm Dmax 13.5 nm VolumePorod 126 nm3

SASDR26 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc5 Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein Synthetic antigen binding fragment tc5 monomer, 49 kDa synthetic construct protein Buffer: phosphate buffered saline, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23 RgGuinier 3.8 nm Dmax 13.5 nm VolumePorod 130 nm3

SASDR36 – Pisum sativum Toc75 Potra domains Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein Buffer: phosphate buffered saline, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23 RgGuinier 2.9 nm Dmax 11.0 nm VolumePorod 60 nm3

SASDR46 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax9 Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein Synthetic antigen binding fragment ax9 monomer, 49 kDa synthetic construct protein Buffer: phosphate buffered saline, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23 RgGuinier 3.8 nm Dmax 13.5 nm VolumePorod 123 nm3

SASDR56 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax14 Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein Synthetic antigen binding fragment ax14 monomer, 48 kDa synthetic construct protein Buffer: phosphate buffered saline, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23 RgGuinier 4.1 nm Dmax 15.5 nm VolumePorod 150 nm3

SASDR66 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax15 Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein Synthetic antigen binding fragment ax15 monomer, 48 kDa synthetic construct protein Buffer: phosphate buffered saline, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23 RgGuinier 3.8 nm Dmax 14.0 nm VolumePorod 123 nm3

SASDR76 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax17 Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein Synthetic antigen binding fragment ax17 monomer, 49 kDa synthetic construct protein Buffer: phosphate buffered saline, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23 RgGuinier 3.4 nm Dmax 12.0 nm VolumePorod 118 nm3