Vibrio cholerae's ToxRS bile sensing system.

Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
Elife 12 (2023 Sep 28)

PMID: 37768326 doi: 10.7554/eLife.88721

Submitted to SASBDB: 2023 Jan 31 Published in SASBDB: 2023 Oct 5

SASDR25 – Periplasmic domain of cholera toxin transcriptional activator ToxR Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6 RgGuinier 1.6 nm Dmax 6.0 nm VolumePorod 16 nm3

SASDR35 – Periplasmic domain of cholera transmembrane regulatory protein ToxS Sample: Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6 RgGuinier 2.3 nm Dmax 7.4 nm VolumePorod 55 nm3

SASDR45 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6 RgGuinier 2.1 nm Dmax 6.6 nm VolumePorod 51 nm3

SASDR55 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS bound to bile salt Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein Bile acid: sodium cholate hydrate monomer, 0 kDa Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Jul 7 RgGuinier 2.4 nm Dmax 6.6 nm VolumePorod 60 nm3