Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme.

Kubiak X, Polsinelli I, Chavas LMG, Fyfe CD, Guillot A, Fradale L, Brewee C, Grimaldi S, Gerbaud G, Thureau A, Legrand P, Berteau O, Benjdia A
Nat Chem Biol (2023 Dec 29)

PMID: 38158457 doi: 10.1038/s41589-023-01493-1

Submitted to SASBDB: 2023 Mar 13 Published in SASBDB: 2024 Feb 15

SASDRS7 – Radical SAM enzyme peptide epimerase in the presence of SAM (S-adenosyl-L-methionine) Sample: Putative peptide biosynthesis protein YydG dimer, 80 kDa Bacillus subtilis (strain … protein Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Nov 29 RgGuinier 2.9 nm Dmax 11.8 nm VolumePorod 115 nm3

SASDRR7 – Radical SAM enzyme peptide epimerase in the presence of SAM (S-adenosyl-L-methionine) and a peptide subtrate Sample: Putative peptide biosynthesis protein YydG dimer, 80 kDa Bacillus subtilis (strain … protein Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Nov 30 RgGuinier 2.9 nm Dmax 12.0 nm VolumePorod 113 nm3