Structural insights into redox signal transduction mechanisms in the control of nitrogen fixation by the NifLA system

Boyer N, Tokmina-Lukaszewska M, Bueno Batista M, Mus F, Dixon R, Bothner B, Peters J
Proceedings of the National Academy of Sciences 120(30) (2023 Jul 17)

doi: 10.1073/pnas.2302732120

Submitted to SASBDB: 2023 Feb 21 Published in SASBDB: 2023 Jul 17

SASDRT5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ADP Sample: Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein Buffer: 50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5 RgGuinier 4.9 nm Dmax 17.9 nm VolumePorod 233 nm3

SASDRU5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ATP Sample: Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein Buffer: 50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5 RgGuinier 4.9 nm Dmax 18.0 nm VolumePorod 225 nm3

SASDRV5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under reducing conditions in the presence of ADP Sample: Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein Buffer: 50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5 RgGuinier 4.9 nm Dmax 18.0 nm VolumePorod 243 nm3

SASDRW5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under reducing conditions in the presence of ATP Sample: Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein Buffer: 50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5 RgGuinier 5.1 nm Dmax 17.8 nm VolumePorod 224 nm3