Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding

Wiedemann C, Whittaker J, Carrillo V, Goretzki B, Dajka M, Tebbe F, Harder J, Krajczy P, Joseph B, Hausch F, Guskov A, Hellmich U
International Journal of Biological Macromolecules :126366 (2023 Aug)

doi: 10.1016/j.ijbiomac.2023.126366

Submitted to SASBDB: 2023 Jun 24 Published in SASBDB: 2023 Jun 26

SASDSY6 – Outer membrane protein MIP (LpMIP) apo state Sample: Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16 RgGuinier 3.1 nm Dmax 10.0 nm VolumePorod 66 nm3

SASDSZ6 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK095 Sample: Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16 RgGuinier 3.1 nm Dmax 10.2 nm VolumePorod 65 nm3

SASDS27 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK236 Sample: Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16 RgGuinier 3.1 nm Dmax 9.4 nm VolumePorod 63 nm3