Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states.

Lin DY, Kueffer LE, Juneja P, Wales TE, Engen JR, Andreotti AH
Elife 12 (2024 Jan 8)

PMID: 38189455 doi: 10.7554/eLife.89489

Submitted to SASBDB: 2023 Apr 18 Published in SASBDB: 2023 Aug 4

SASDRB9 – Bruton's Tyrosine Kinase - full-length Sample: Tyrosine-protein kinase BTK monomer, 77 kDa Mus musculus protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 2% v/v glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 9 RgGuinier 4.1 nm Dmax 19.1 nm VolumePorod 118 nm3

SASDRC9 – Bruton's Tyrosine Kinase - full-length with A384P, S386P, T387P, A388P and L390F mutations Sample: Tyrosine-protein kinase BTK (A384P, S386P, T387P, A388P, L390F) monomer, 77 kDa Mus musculus protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 2% v/v glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 9 RgGuinier 3.9 nm Dmax 15.2 nm VolumePorod 107 nm3

SASDRD9 – Bruton's Tyrosine Kinase - SH3-SH2-kinase domain Sample: Tyrosine-protein kinase BTK (SH3-SH2-kinase domains) monomer, 52 kDa Mus musculus protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 2% v/v glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 9 RgGuinier 2.4 nm Dmax 7.5 nm VolumePorod 68 nm3

SASDRE9 – Bruton's Tyrosine Kinase - SH3-SH2-kinase domain with A384P, S386P, T387P, A388P and L390F mutations Sample: Tyrosine-protein kinase BTK (SH3-SH2-kinase domain A384P, S386P, T387P, A388P, L390F) monomer, 53 kDa Mus musculus protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 2% v/v glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 9 RgGuinier 2.5 nm Dmax 7.8 nm VolumePorod 71 nm3