X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization.

Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
Acta Crystallogr D Struct Biol (2023 Oct 1)
PMID: 37712436
doi: 10.1107/S2059798323006514 		Submitted to SASBDB: 2023 Apr 19
Published in SASBDB:

SASDR39 – MBP-, SUMO-fused heterodimeric SEPT6-SEPT7 septin coiled coil (MBP-SEPT6c+SUMO-SEPT7c)

Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP)Septin-7 (C-terminal domain of SEPT7 fused to SUMO) experimental SAS data
MULTIFOXS model
Sample: Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP) monomer, 55 kDa Homo sapiens protein
Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
RgGuinier 5.4 nm
Dmax 21.5 nm
VolumePorod 142 nm3

SASDR49 – MBP-, SUMO-fused heterodimeric SEPT8-SEPT7 septin coiled coil (MBP-SEPT8c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-8 (C-terminal domain of SEPT8 fused to MBP) experimental SAS data
MULTIFOXS model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-8 (C-terminal domain of SEPT8 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
RgGuinier 5.3 nm
Dmax 19.9 nm
VolumePorod 140 nm3

SASDR59 – MBP-, SUMO-fused heterodimeric SEPT10-SEPT7 septin coiled coil (MBP-SEPT10c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-10 (C-terminal domain of SEPT10 fused to MBP) experimental SAS data
DAMMIN model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-10 (C-terminal domain of SEPT10 fused to MBP) monomer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
RgGuinier 5.7 nm
Dmax 21.7 nm
VolumePorod 154 nm3

SASDR69 – MBP-, SUMO-fused heterodimeric SEPT11-SEPT7 septin coiled coil (MBP-SEPT11c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-11 (C-terminal domain of SEPT11 fused to MBP) experimental SAS data
MULTIFOXS model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-11 (C-terminal domain of SEPT11 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
RgGuinier 5.2 nm
Dmax 20.9 nm
VolumePorod 139 nm3

SASDR79 – MBP-, SUMO-fused heterodimeric SEPT14-SEPT7 septin coiled coil (MBP-SEPT14c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-14 (C-terminal domain of SEPT14 fused to MBP) experimental SAS data
MULTIFOXS model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-14 (C-terminal domain of SEPT14 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
RgGuinier 5.1 nm
Dmax 20.3 nm
VolumePorod 143 nm3