Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity.

Houry D, Raasakka A, Ferrario E, Niere M, Bifulco E, Kursula P, Ziegler M
J Struct Biol :108004 (2023 Jul 24)

PMID: 37495196 doi: 10.1016/j.jsb.2023.108004

Submitted to SASBDB: 2023 Mar 26 Published in SASBDB: 2023 Jul 27

SASDRZ7 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT) Sample: Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20 RgGuinier 3.3 nm Dmax 12.9 nm VolumePorod 181 nm3

SASDR28 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT) in the presence of nicotinamide Sample: Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinamide, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20 RgGuinier 3.2 nm Dmax 12.8 nm VolumePorod 166 nm3

SASDR38 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT) in the presence of nicotinic acid Sample: Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinic acid, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20 RgGuinier 3.3 nm Dmax 12.9 nm VolumePorod 180 nm3

SASDR48 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT) in the presence of phosphoribosyl pyrophosphate Sample: Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM phosphoribosyl pyrophosphate, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20 RgGuinier 3.3 nm Dmax 12.9 nm VolumePorod 173 nm3

SASDR58 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) Sample: Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20 RgGuinier 3.2 nm Dmax 11.0 nm VolumePorod 159 nm3

SASDR68 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of nicotinamide Sample: Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinamide, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20 RgGuinier 3.2 nm Dmax 10.9 nm VolumePorod 151 nm3

SASDR78 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of nicotinic acid Sample: Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinic acid, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20 RgGuinier 3.3 nm Dmax 11.2 nm VolumePorod 152 nm3

SASDR88 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of phosphoribosyl pyrophosphate Sample: Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM phosphoribosyl pyrophosphate, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20 RgGuinier 3.2 nm Dmax 11.4 nm VolumePorod 154 nm3